Irradiation of Animal Sperm with Radio Waves:
Results and Prospects Summary

Experiments on irradiation of the animal sperm with the low intensity electromagnetic fields of millimeter waves proved the availability of the stimulating effect as to spermatozoa mobility and increase of their life. This technique can be applied for transplantation and cloning.

Setting up the problem

Well known is the significant decrease of biological activity of spermatozoa during the process of their technological treatment in artificial fecundating of animals. The animals' sperm is characterized by a high variability of individual sensitivity to effects of the low temperature of cryogenic treatment. To correct a low quality sperm it is proposed to irradiate it with a non-radioactive electromagnetic field.

Material and equipment

In the experiment we used the serial sperm-doses made of sperm-material of studbulls of Israel breed. A multimeter wave generator with rearranging frequency and a conventional mobility measuring device were used.

Techniques

A sperm-dose taken out of the Dewar vessel was put into a heating chamber. Then after defrosting the sperm-dose was put into the mobility measuring device. Next the sperm-dose was subjected to irradiation by the millimeter wave generator. After the irradiation was over the sperm-dose was put into the mobility measuring device again. The experiments were conducted for two values of the irradiation time.

Results

All in all 10 experiments have been conducted of which 7 ones of 5 seconds' irradiation and 3 experiments of 10 seconds' irradiation. The measurements obtained are characterized by the following values.

With the 5 seconds' sperm irradiation the expectation of absolute values equals 716 with the mean square deviation 3377 and the range of the sample 254. The expectation of relative values of the post-experimental measurements equals +144 with the mean square deviation 3036 and the range of the sample 191.

The expectation of relative values of the post-experimental measurements with the sperm irradiation for 10 seconds equals -154. In this case, it is worth noting that with the 5 or 10 seconds' sperm irradiation one can observe either the mobility stimulation in all 7 experiments or mobility suppression of spermatozoa in 3 experiments.

Discussion of results

The experiments carried out have proved the presence of the radioelectronic resonance within biological systems when being irradiated with low intensity millimeter waves [1,2,3]. Some authors believe that the most important point of application of such irradiation action is the biological membrane because the low intensity irradiation results in acceleration or suppression of the active transportation, in changing the permeability of biological membranes on account of the irradiation effect on the conformation of proteins in the cell membrane and the protein component of ATP-synthaze complex. The results of the conducted experiments make possible the correction of a low quality sperm by treating it with millimeter irradiation. One should take into account the possibility of treating by the mentioned irradiation of some biological objects in transplanting and cloning.

Acknowledgment

I wish to express my deep gratitude to Professor B. Bartov, Dr. N. Zabludovsky from Bar-Ilan University, Dr. E. Aizenbood from Vulcan Center and to Mr. D. Kelai, President of National Committee for Breeding of Israel Holstein Cattle who gave me a helpful hand in conducting the experiments.

References

• Grundler W., Kaiser F., Welleczek J. (1992): Mechanisms of electromagnet interaction with cellular systems, Naturwissenschaften 79:551-559
• Belyaev I.V.(1992): Some biophysical aspects of genetic effect of low intensity millimeter waves, Bioelectrochemical Bioengineering, 27:11-18
• Royavin M.A., Ziskin M.C. (1995): Effect of Millimeter Waves on Survival of UVC-Exposed Esherchia coli, Bioelectromagnetics 16:188-196